He offered to resign after comments had made in an interview after Tuesday night's Wycombe came in which he said Wise wouldn't be leaving Swindon.
"In the fullness of time maybe in a couple of years time he may go somewhere else but at the moment he's told us he wants to stay with this club, Swindon Town Football Club, to finish the job he intended from the very beginning."
but Dennis Wise wasn't happy with Holt repeating that in public, so after 6 years with Swindon Bob Holt has resigned.
He's the second Swindon director to go in a week after Bill Power had to step down last week after been injured in a plane crash in August which he needs another 18 months to recover from.